The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR products were digested and cloned into a pET21a+ vector employing NdeI and XhoI restriction enzymes. A point mutation was released by the short change web-site-directed https://wille433ozk5.blogoxo.com/profile